Conversion of AFLP bands into high-throughput DNA markers
نویسندگان
چکیده
منابع مشابه
A new and versatile method for the successful conversion of AFLP markers into simple single locus markers.
Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the conversion of AFLP markers from complex fingerprints into simple single locus assays is perceived as problematic because DNA sequence information is required for the design of new locus-specific PCR primers. In a...
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Amplified fragment length polymorphism (AFLP) analysis is currently the most powerful and efficient technique for the generation of large numbers of anonymous DNA markers in plant and animal genomes. We have developed a protocol for high-throughput AFLP analysis that allows up to 70,000 polymorphic marker genotype determinations per week on a single automated DNA sequencer. This throughput is b...
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Targeted resequencing of genomic DNA from organisms such as humans is an important tool enabling experimental access to variation within the species and between similar species. Taking full advantage of the reference genome sequences in designing robust, specific PCR assays and using stringent conditions, resequencing can be done efficiently without purification of the PCR product. By using a 1...
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staining. However, these visualization methods require either expensive or hazardous radioactive chemicals and Microsatellite DNA markers are widely used in genetic research. are time-consuming. Electrophoresis with MetaPhor Their use, however, can be costly and throughput is sometimes limited. The objective of this paper is to introduce a simple, low-cost, highagarose gels (Cambrex Corporation...
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ژورنال
عنوان ژورنال: Molecular Genetics and Genomics
سال: 2001
ISSN: 1617-4615,1617-4623
DOI: 10.1007/s004380000418